A research team has identified a progenitor population of cells that may serve as a carrier of mutations involved in the development of both high-risk and low-risk Langerhans cell histiocytosis (LCH). These findings were reported in Blood.
The investigators explained that LCH is associated with activating mutations involved in the mitogen-activated protein kinase (MAPK) pathway. According to the researchers, LCH generally exists as either a high-risk, multisystem form that appears to arise from the bone marrow or as a low-risk, single-system (SS) form that may emerge from circulating or tissue-specific cells.
The research team hypothesized, based on prior research, that the Lin–CD34+c-Kit+Flt3+ myeloid progenitor population may possess a driver mutation, such as a BRAF variant, associated with LCH in both high-risk and low-risk patient groups.
In their analysis, samples were obtained from blood, bone marrow, and/or from LCH lesions from patients with LCH. Blood and bone marrow samples were also obtained from hematopoietic stem cell donors. A total of 27 samples were analyzed from patients with LCH, and 43 samples were analyzed from donors.
Cells were isolated through flow cytometry or immunohistochemistry. Next-generation sequencing or droplet digital polymerase chain reaction was performed for detection of BRAFV600E, BRAFV600D, or BRAFWT sequences. The researchers also cultured and aimed to differentiate Lin–CD34+c-Kit+Flt+ cells into dendritic cell (DC) or Langerhans cell (LC)-like groups.
Samples from both high-risk and low-risk patients showed elevated levels of CD34+c-Kit+Flt3+ cells compared with tissue from donors. These cells originating from both high-risk and low-risk patients were able to serve as progenitors of both DC and LC-like populations, and BRAF mutations were detected across multiple myeloid cell types in LCH lesions of low-risk patients.
“The presence of mutated progenitor cells in lesions of SS LCH patients and mutated myeloid cells in both blood and lesions of low-risk LCH patients (our data) suggest that the mechanism underlying low-risk and high-risk LCH pathogenesis may be the same,” the researchers wrote.
One clinical implication that the researchers identified was that localized treatment given to patients with a single LCH lesion may be insufficient, and that systemic therapy may be required.
Xiao Y, van Halteren A, Lei X, et al. Bone marrow-derived myeloid progenitors as driver mutation carriers in high- and low-risk Langerhans cell histiocytosis. Blood. Published online August 4, 2020. doi:10.1182/blood.2020005209